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Isolation and Characterization of Medicinally Important Marine Penicillium Isolates

Isolation and Characterization of Medicinally Important Marine Penicillium Isolates

Ibrar Khan1,2,3,*, Sadia Qayyum2, Shehzad Ahmed2, Kashif Syed Haleem2, Mujaddad-ur-Rehman1, Guang-Lei Liu3 and Zhen-Ming Chi3

1Department of Microbiology, Abbottabad University of Science & Technology, 22010 Pakistan
2Department of Microbiology, Hazara University, 21300 Mansehra, Pakistan
3UNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao 266003, China

* Corresponding author: me_abrarkhan@yahoo.com

Figure 1

The colony morphology of the strain F1 (Penicillium viticola) F2 (Penicillium restrictum) (a, front; b, back; c, mycelium and the chains of its conidia).

Figure 2

The morphologies of the colony of the strain F8 (Penicillium ruben) (a, front; b, back; c, mycelium and the chains of its conidia and the strain) F10 (Penicillium implicatum) (d, front; e, back; f, mycelium and the chains of its conidia).

Figure 3

The morphologies of the colony of the strain F11 (Penicillium piceum) (a, front b, back and c, mycelium and the chains of its conidia; the strain F12 (Penicillium oxalicum) (d, front; e, back; f, mycelium and the chains of its conidia).

Figure 4

The morphologies of the colony of the strain F15 (Penicillium sumatrense) (a, front; b, back; c, mycelium and the chains of its conidia; the srain F16 (Penicillium vinaceum) (d, front; e, back; f, mycelium and the chains of its conidia).

Figure 5

Genomic DNAs of the fungal isolates; lane M: 1 kb DNA ladder, Lane F1, F2 F8, F10, F11, F12, F15, and F16 are the genomic DNAs of them.

Figure 6

Resolving pattern of PCR amplified ITS sequences on 1% Agarose gel; Lanes F1, F2, F8, F10, F11, F12, F15, and F16 showed PCR products of the fungal isolates and Line M: DNA ladder, fragment size from top to bottom is 2000bp, 1000bp, 750bp, 500bp, 250bp and100bp.

Figure 7

The phylogenetic tree of the all fungal isolates and other Penicillium species relatives based on a neighbor-joining analysis of ITS sequences. Aspergillus flavus CBS 260.73 was used as out group.

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