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Comparative Study on Induction of Apoptosis in A549 Human Lung Adenocarcinoma Cells by C2 Ceramide or Ceranib-2

Comparative Study on Induction of Apoptosis in A549 Human Lung Adenocarcinoma Cells by C2 Ceramide or Ceranib-2

Gökhan Kuş1,*, Hatice Mehtap Kutlu2, Djanan Vejselova2 and Emre Comlekci3 

1Department of Health Programme, Faculty of Open Education, Anadolu University, Eskişehir, Turkey
2Department of Biology, Faculty of Science, Anadolu University, Eskişehir, Turkey
3Department of Molecular Biology and Genetics, Graduate School of Sciences, Bilecik Şeyh Edebali University, Bilecik, Turkey

 

Fig 1

Apoptosis quantification of A549 cells treated with C2 ceramide for 24 h. A, Untreated A549 cells; B, C2 Ceramide treated A549 cells.

Fig 2

Apoptosis quantification of A549 cells treated with ceranib-2 for 24 h. A, Untreated A549 cells; B, Ceranib-2 treated A549 cells.

Fig 3

Analysing the mitochondrial membrane potential changes of A549 cells. A, Untreated A549 cells; B, A549 cells exposed to C2 ceramide.

Fig 4

Mitochondrial membrane potential changes of A549 cells. A, Untreated A549 cells: B, A549 cells exposed to ceranib-2.

Fig 5

Ultrastructural changes of A549 cells. A, Untreated A549 cells: ∆, normal cell membrane; →, normal nuclear membrane. B, A549 cells exposed to C2 ceramide for 24 h: →, autophagic vacuols; ∆, loss of cristae. C, chromatin condensation and ◊, is circular shaped cell.

Fig 6

Ultrastructural changes of A549 cells. A, Untreated A549 cells: →, undamaged cell membrane; ∆, normal nuclear membrane. B, A549 cells exposed to ceranib-2 for 24 h. ◊, fragmented nucleus. C, →, Circular shaped cell and membrane blebbing. D, ◊, Lipid droplets in cells. E, ◊, DNA disintegration, →, holes in cell.

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