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Assessing the Validity of Fasciola hepatica ELISA Test for Immunodiagnosis of Small Ruminant Fasciolosis in Pothwar Region, Pakistan

Assessing the Validity of Fasciola hepatica ELISA Test for Immunodiagnosis of Small Ruminant Fasciolosis in Pothwar Region, Pakistan

Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2

1Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan
2Department of Zoology, Faculty of Sciences, PMAS-Agriculture University, Rawalpindi-46300, Pakistan

Fig 1
 Mean DRG Units (DU) obtained with the DRG bovine F. hepatica IgG ELISA test in fascioliasis positive sera collected from small ruminants grazing in sub-tropical Punjab, Pakistan. The results were negative if DU<13, and positive if DU> 13 (dotted line).
Fig 2

Mean DRG Units (DU) obtained with the DRG bovine F. hepatica IgG ELISA test in small ruminants A) animals infected with parasites other than fascioliasis B) and negative control sera. The results were negative if DU<13, and positive if DU>13(dotted line).

Fig 3

The Receiver-operating characteristic (ROC) curve representing excellent tests results (ROC area =0.998). The accuracy of the test (95.6%) separated the group being tested into those with and without the disease. Accuracy is measured by the area under the ROC curve. An area of 1 represents a perfect test.

Fig 4

Mean DRG Units (DU) obtained with the DRG bovine F. hepatica IgG ELISA test in different breeds of A) sheep and B) goats. The results were negative if DU<13, and positive if DU>13 (dotted line).

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