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Assessing the Validity of Fasciola hepatica ELISA Test for Immunodiagnosis of Small Ruminant Fasciolosis in Pothwar Region, Pakistan

Assessing the Validity of Fasciola hepatica ELISA Test for Immunodiagnosis of Small Ruminant Fasciolosis in Pothwar Region, Pakistan

Kiran Afshan1*, Sarwat Jahan1 and Mazhar Qayyum2

1Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan
2Department of Zoology, Faculty of Sciences, PMAS-Agriculture University, Rawalpindi-46300, Pakistan

ABSTRACT

To improve the diagnostic efficacy of animal fascioliasis caused by Fasciola hepatica and Fasciola gigantica in Pakistan, we first time evaluated the diagnostic accuracy of commercially available bovine F. hepatica enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against fascioliasis in sera of small ruminants. The result of current study indicated diagnostic accuracy of test 95.6%, while sensitivity and specificity of this assay for small ruminants was 97.83% (95% CI: 92.35% to 99.67%) and 93.75% (95% CI: 87.54% to 97.44%) respectively. In field this test indicated significant (p<0.05) values for sheep as compared to goat indicating high risk of infection in sheep. The current study showed that commercial bovine F. hepatica ELISA test is effective in Pakistan for small ruminants.
 

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